Isolation and Characterization of Antimicrobial Compound from Marine Streptomyces hygroscopicus BDUS 49

A total of sixty eight actinomycetes were isolated from near sea shore marine environment locations of Bigeum Island, South West coast of South Korea. The majority of these isolates were assigned to the genus Streptomyces of which one isolate showing broad spectrum of antimicrobial was on the basis of their morphological, physiological and biochemical properties. Phylogenetic analysis of the 16S rRNA gene sequence of one isolate e showed that strain was Streptomyces hygroscopicus BDUS 49 and it formed a distinct clad within the Streptomyces 16S rRNA gene tree closely related to Streptomyces hygroscopicus. This strain possessed a broad spectrum antimicrobial activity against Gram-positive,-& Gram-negative bacteria and fungi. The UV spectra of the active compounds in ethyl acetate showed peaks at between 200 to 295 nm. The bioactive region was detected on the TLC plate (R 0.40). The structure of the bioactive components was further f determined using FTIR, MS, C NMR and H NMR. The molecular formula of the given partially purified 13 1 compound being identified as 7, demethoxy rapamycin and their molecular formula derived as C H NO +NA (MW 905.12). 50 75 12